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KMID : 0624620200530020106
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BMB Reports
2020 Volume.53 No. 2 p.106 ~ p.111
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PEP-1-GLRX1 Protein Exhibits Anti-Inflammatory Effects by Inhibiting the Activation of MAPK and NF-κB Pathways in Raw 264.7 Cells
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Shin Min-Jea
Kim Dae-Won
Choi Yeon-Joo
Cha Hyun-Ju
Lee Sung-Ho
Lee Sung-Hou
Park Jin-Seu
Han Kyu-Hyung
Eum Won-Sik
Choi Soo-Young
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Abstract
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Glutaredoxin 1 (GLRX1) has been recognized as an important regulator of redox signaling. Although GLRX1 plays an essential role in cell survival as an antioxidant protein, the function of GLRX1 protein in inflammatory response is still under investigation. Therefore, we wanted to know whether transduced PEP-1-GLRX1 protein inhibits lipopolysaccharide (LPS)- and 12-O-tetradecanoyl phorbol-13-acetate (TPA)-induced inflammation. In LPS-exposed Raw 264.7 cells, PEP-1-GLRX1 inhibited cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), activation of mitogen activated protein kinases (MAPKs) and nuclear factor-kappaB (NF-κB) expression levels. In a TPA-induced mouse-ear edema model, topically applied PEP-1-GLRX1 transduced into ear tissues and significantly ameliorated ear edema. Our data reveal that PEP-1-GLRX1 attenuates inflammation in vitro and in vivo, suggesting that PEP-1-GLRX1 may be a potential therapeutic protein for inflammatory diseases.
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KEYWORD
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Inflammation, MAPK, NF-κB, PEP-1-GLRX1, Protein therapy
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